製品の概要

  • 製品名Anti-MED10 antibody
  • 製品の詳細
    Rabbit polyclonal to MED10
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MED10.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; HeLa; Jurkat; MCF7; Caco2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.

Use with paraformaldehyde fixed cells.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 16 kDa).

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 配列類似性Belongs to the Mediator complex subunit 10 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • L6 antibody
    • med10 antibody
    • MED10_HUMAN antibody
    • Mediator complex subunit 10 antibody
    • Mediator of RNA polymerase II transcription subunit 10 antibody
    • Mediator of RNA polymerase II transcription, subunit 10 homolog antibody
    • NUT2 antibody
    • Transformation-related gene 17 protein antibody
    • Transformation-related gene 20 protein antibody
    • Transformation-related protein 17 antibody
    • Transformation-related protein 20 antibody
    • TRG-17 antibody
    • TRG-20 antibody
    • TRG17 antibody
    • TRG20 antibody
    see all

Anti-MED10 antibody 画像

  • ab110786 (1/200) staining MED10 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please see Abreview.

    See Abreview

  • All lanes : Anti-MED10 antibody (ab110786) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,21 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110786 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-MED10 antibody (ab110786) 使用論文

ab110786 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"