製品の概要

  • 製品名
    Anti-MEC4 antibody
  • 製品の詳細
    Mouse polyclonal to MEC4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Caenorhabditis elegans
  • 免疫原

    Fusion protein:

    FFEPGFARCLCGSQGSSEQEDKDEEKEEELLETTTKKVFNINDADEEWDG MEEYDNEHYENYDVEATTGMNMMEECQSERTKFDEPTGFDDRCICAFDRS

    , corresponding to amino acids 232/322 of Caenorhabditis elegans MEC4

  • 特記事項
    Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Constituents: 50% Glycerol
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 87 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性
    MEC4 is a probable sodium channel subunit. It may be needed for mechanosensory transduction (touch sensitivity). The channel is probably composed of at least the mec-2, mec-4, mec-6 and mec-10 subunits. MEC4 is coexpressed with mec-6 in touch-cell axons. Mutations in MEC4 results in the degeneration of a small set of neurons which typically swell to several time their normal diameter before they disappear, presumably due to lysis.
  • 細胞内局在
    Integral membrane protein.
  • 参照データベース
    • 別名
      • Degenerin mec 4 antibody
      • mec 13 antibody
      • Mechanosensory abnormality protein 4 antibody
      • T01C8.7 antibody

    画像

    • All lanes : Anti-MEC4 antibody (ab22184) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 87 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Chen Y  et al. MEC-10 and MEC-19 Reduce the Neurotoxicity of the MEC-4(d) DEG/ENaC Channel in Caenorhabditis elegans. G3 (Bethesda) 6:1121-30 (2016). IF . Read more (PubMed: 27172609) »
    • Chen Y  et al. Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. Mol Biol Cell 27:1272-85 (2016). IF . Read more (PubMed: 26941331) »

    See all 3 Publications for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab22184.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録