製品の概要

  • 製品名
    Anti-Measles nucleoprotein antibody [3E1]
    Measles nucleoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3E1] to Measles nucleoprotein
  • 特異性
    Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • アプリケーション
    適用あり: WB, Indirect ELISAmore details
  • 免疫原

    Recombinant full length protein.

  • ポジティブ・コントロール
    • recombinant measles NP
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    3E1
  • ミエローマ
    NS0/1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000.
Indirect ELISA 1/1000 - 1/10000.

ターゲット情報

  • 関連性
    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • 細胞内局在
    Virion. Host cytoplasm.
  • 別名
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

画像

  • Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.

プロトコール

参考文献

This product has been referenced in:
  • Sun Q  et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). Read more (PubMed: 23794264) »
  • Wang FM  et al. Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling. Bone 53:269-76 (2013). Read more (PubMed: 23262029) »

See all 3 Publications for this product

レビューと Q&A

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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