製品の概要

  • 製品名
    Anti-Measles nucleoprotein antibody [2D7]
    Measles nucleoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2D7] to Measles nucleoprotein
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 免疫原

    Recombinant measles NP

  • ポジティブ・コントロール
    • recombinant measles NP
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    2D7
  • ミエローマ
    NS0/1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Detects a band of approximately 52.5 kDa.

ターゲット情報

  • 関連性
    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • 細胞内局在
    Virion. Host cytoplasm.
  • 別名
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

画像

  • Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

    Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

プロトコール

参考文献

This product has been referenced in:
  • Lo MK  et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). Read more (PubMed: 28262699) »
  • Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). Read more (PubMed: 21971444) »

See all 5 Publications for this product

レビューと Q&A

Thank you for your enquiry. The molecular weight of the protein (measles nucleocapsid protein) is 52.5 kDa. The size of the orange marker protein is 70 kDa. Please contact us again if you have additional questions.

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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Thank you for the information that you have provided and I'm sorry to hear that your customer is experiencing difficulty with ab9882. We don't have lot 200502 in stock anymore. This is the first complaint that we have received concerning this antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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