製品の概要

  • 製品名Anti-MDMX (phospho S403) antibody
    MDMX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MDMX (phospho S403)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic phospho-peptide corresponding to the region around Serine 403 of Human MDMX (SwissProt O15151)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. Detects a band of approximately 80 kDa.

ターゲット情報

  • 機能Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • 組織特異性Expressed in all tissues tested with high levels in thymus.
  • 配列類似性Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • ドメインRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • 翻訳後修飾Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX (phospho S403) antibody 画像

  • All lanes : Anti-MDMX (phospho S403) antibody (ab70236) at 0.13 µg/ml

    Lane 1 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (2hrs at 200 ng/ml)
    Lane 2 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (0.5hrs at 200 ng/ml)
    Lane 3 : 293T whole cell lysate with HA tagged-MDMX S403A mutant
    Lane 4 : 293T whole cell lysate with HA tagged-MDMX (wild-type) and NCS (2hrs at 200 ng/ml)
    Lane 5 : 293T whole cell lysate with HA tagged-MDMX(wild-type) and NCS (0.5hrs at 200 ng/ml)
    Lane 6 : 293T whole cell lysate with HA tagged-MDMX (wild-type)
    Lane 7 : mock transfected 293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique

    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-MDMX (phospho S403) antibody (ab70236) 使用論文

ab70236 has not yet been referenced specifically in any publications.

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