製品の概要

  • 製品名Anti-MDMX (phospho S367) antibody [15]
    MDMX 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [15] to MDMX (phospho S367)
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence of Human MDXM protein surrounding phospho-Ser367.

  • ポジティブ・コントロール
    • MCF cells reincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • 組織特異性Expressed in all tissues tested with high levels in thymus.
  • 配列類似性Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • ドメインRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • 翻訳後修飾Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX (phospho S367) antibody [15] 画像

  • All lanes : Anti-MDMX (phospho S367) antibody [15] (ab122926) at 1 µg/ml

    Lane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)
    Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour
    Lane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour
    Lane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour
    Lane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour
    Lane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour
    Lane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour
    Lane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour
    Lane 9 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour


    Predicted band size : 55 kDa

Anti-MDMX (phospho S367) antibody [15] (ab122926) 使用論文

This product has been referenced in:
  • Okamoto K  et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). Read more (PubMed: 16227609) »

See 1 Publication for this product

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