製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MDH2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 40-289 of Human MDH2 (NP_005909).

  • ポジティブ・コントロール
    • 293T whole cell lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

ターゲット情報

画像

  • Anti-MDH2 antibody (ab96193) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 36 kDa
  • All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution

    Lane 1 : Mouse C2C12 whole cell lysate
    Lane 2 : Mouse C2C12 whole cell lysate on day 1 of differentiation
    Lane 3 : Mouse C2C12 whole cell lysate on day 2 of differentiation

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Anonymous Abreview.

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Naseri NN  et al. Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients. PLoS One 11:e0160384 (2016). WB . Read more (PubMed: 27611087) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
20 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Feb 26 2011

Application
Western blot
Sample
Rat Cell lysate - other (L6 myotubes)
Loading amount
20 µg
Specification
L6 myotubes
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Feb 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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