製品の概要

  • 製品名Anti-MDH2 antibody [2F5AF8]
    MDH2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2F5AF8] to MDH2
  • アプリケーション適用あり: ICC/IF, In-Cell ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Tissue, cells or virus corresponding to Pig MDH2. Purified protein from porcine heart.

  • ポジティブ・コントロール
    • Fibroblast cells Human heart homogenate Rat liver homogenate Mouse liver homogenate HepG2 whole cell lysate
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent dilution.

ターゲット情報

Anti-MDH2 antibody [2F5AF8] 画像

  • Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

Anti-MDH2 antibody [2F5AF8] (ab110317) 使用論文

ab110317 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"