製品の概要

  • 製品名
    Anti-MDC1 antibody [MDC1-50]
    MDC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MDC1-50] to MDC1
  • アプリケーション
    適用あり: WB, IP, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Recombinant fragment, corresponding to amino acids 2-200 of Human MDC1

  • ポジティブ・コントロール
    • WB: total cell extract of G361 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)
    This antibody is a purified Mouse Immunoglobulin.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MDC1-50
  • ミエローマ
    NS1
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 226 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 組織特異性
    Highly expressed in testis.
  • 配列類似性
    Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • ドメイン
    Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻訳後修飾
    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 細胞内局在
    Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

画像

  • ab50003 staining MDC1 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using NP40, blocked with 3% BSA for 3 hours at 25°C and then incubated with ab50003 at a 1/200 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated sheep anti-mouse IgG polyclonal used at a 1/500 dilution.

    MDC1 appears as green foci, the nucleus has been stained with DAPI (blue).

    See Abreview

参考文献

This product has been referenced in:
  • Pederiva C  et al. Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ 23:1648-57 (2016). Read more (PubMed: 27315300) »
  • Coucoravas C  et al. Phosphorylation of the Cajal body protein WRAP53ß by ATM promotes its involvement in the DNA damage response. RNA Biol N/A:1-10 (2016). Human . Read more (PubMed: 27715493) »

See all 7 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (smooth muscle cells)
Specification
smooth muscle cells
Fixative
Acetone
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - NP40
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

投稿 Mar 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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