製品の概要

  • 製品名Anti-MDC1 antibody
    MDC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MDC1
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding a region between residues 1375 and 1425 of Human MDC1 (NP_055456.1)

  • ポジティブ・コントロール
    • Human skin tumour tissue. Human breast adenocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
    • 組織特異性Highly expressed in testis.
    • 配列類似性Contains 2 BRCT domains.
      Contains 1 FHA domain.
    • ドメインTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
    • 翻訳後修飾Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
    • 細胞内局在Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Homologue to Drosophila photoreceptor protein calphotin antibody
      • MDC 1 antibody
      • Mdc1 antibody
      • MDC1_HUMAN antibody
      • Mediation of DNA damage checkpoint 1 antibody
      • Mediator of DNA damage checkpoint 1 antibody
      • Mediator of DNA damage checkpoint protein 1 antibody
      • NFBD 1 antibody
      • NFBD1 antibody
      • Nuclear factor with BRCT domains 1 antibody
      • Nuclear Factor with BRCT Domains Protein 1 antibody
      see all

    Anti-MDC1 antibody 画像

    • Detection of Human MDC1 by Immunohistochemistry.
      Sample: FFPE section of Human skin tumour.
      Antibody: ab84079 used at a dilution of 1/250.
      Detection: DAB staining using anti Rabbit IHC antibody at a dilution of 1/100.
    • Detection of Human MDC1 by Immunohistochemistry.
      Sample: FFPE section of Human breast adenocarcinoma.
      Antibody: ab84079 used at a dilution of 1/250.
      Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.

    Anti-MDC1 antibody (ab84079) 使用論文

    ab84079 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"