The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 250 kDa).
機能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
ドメインTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
翻訳後修飾Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
細胞内局在Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
Homologue to Drosophila photoreceptor protein calphotin antibody
MDC 1 antibody
Mediation of DNA damage checkpoint 1 antibody
Mediator of DNA damage checkpoint 1 antibody
Mediator of DNA damage checkpoint protein 1 antibody
NFBD 1 antibody
Nuclear factor with BRCT domains 1 antibody
Nuclear Factor with BRCT Domains Protein 1 antibody
Anti-MDC1 antibody 画像
Western blot - MDC1 antibody (ab13858)
Predicted band size : 250 kDa
Western blot analysis of MDC1 in 30 ugs of Jurkat cell lysate using ab 13858 at 1/1000 dilution.
Anti-MDC1 antibody (ab13858) 使用論文
This product has been referenced in:
Ismail IA et al. Genistein-induced neuronal apoptosis and G2/M cell cycle arrest is associated with MDC1 up-regulation and PLK1 down-regulation. Eur J Pharmacol575:12-20 (2007).
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