製品の概要

  • 製品名Anti-MDC1 antibody
    MDC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MDC1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, which represents a portion of the human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (LocusLink ID 9656).

  • ポジティブ・コントロール
    • Tested with HeLa cells and HEK 293 cells.
  • 特記事項

    In response to recent feedback about mouse species we no longer guarantee this anymore. Please do contact our scientific support team for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were affinity purified using the peptide immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2500. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).

ターゲット情報

  • 機能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 組織特異性Highly expressed in testis.
  • 配列類似性Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • ドメインTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻訳後修飾Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 細胞内局在Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody 画像

  • ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
    The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


  • Predicted band size : 220 kDa

    Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

    Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

  • ab11169 staining MDC1 in human cervical cancer cells (HeLa) by ICC/IF (immunocytochemistry/immunofluorescence - left image). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 25°C. The primary antibody (1/200) was incubated with the sample for 12 hours at 4°C. An Alexa Fluor 594®-conjugated Chicken anti-rabbit polyclonal (1/2000) was used as the secondary. The nuclei were stained with DAPI (right image).

    See Abreview

  • Immunofluorescence analysis of A549 cells before (left) and after ionizing radiation (right), staining MDC1 with ab11169.

    Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at room temperature. Cells were incubated with primary antibody for 1 hour at room temperature. A Cy2®-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-MDC1 antibody (ab11169) 使用論文

This product has been referenced in:
  • Leung JW  et al. Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A ubiquitination and DNA damage signaling. PLoS Genet 10:e1004178 (2014). Human . Read more (PubMed: 24603765) »
  • Liang H  et al. Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing. Nat Commun 5:4048 (2014). WB ; Human . Read more (PubMed: 24893992) »

See all 16 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Treatment IR
Specification HEK293
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 26 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.2% Triton X-100
Specification HeLa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Oct 06 2016

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Cell lysate - other (MEF)
Specification MEF
Treatment with/without IR 20Gy 1hr
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 25 2015

Application IHC - Wholemount
Sample Mouse Embryo (stage e6.5)
Specification stage e6.5
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投稿 Dec 15 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Permeabilization Yes - 0.5% Triton CSK buffer pre-extraction
Fixative Formaldehyde
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投稿 Mar 31 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cervical cancer)
Specification cervical cancer
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hek293, MCF-7 and rat Vascular Smooth Muscle Cells)
Loading amount 20 µg
Specification Hek293, MCF-7 and rat Vascular Smooth Muscle Cells
Gel Running Conditions Reduced Denaturing (5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Sep 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"