製品の概要

  • 製品名Anti-MDC antibody
    MDC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MDC
  • アプリケーション適用あり: Sandwich ELISA, Neutralising, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hMDC(human Macrophage-Derived Chemokine)

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプunknown
  • 軽鎖の種類unknown
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

This antibody used as the capture antibody can be paired with ab83129 as the detection antibody allowing the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.

Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19318474

ターゲット情報

  • 機能May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • 組織特異性Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A 152E5.1 antibody
    • ABCD 1 antibody
    • ABCD1 antibody
    • C C motif chemokine 22 antibody
    • CC chemokine STCP 1 antibody
    • CC chemokine STCP-1 antibody
    • ccl 22 antibody
    • Ccl22 antibody
    • CCL22_HUMAN antibody
    • Chemokine (C C motif) ligand 22 antibody
    • DC/B CK antibody
    • DCBCK antibody
    • Macrophage-derived chemokine antibody
    • MDC antibody
    • MDC(1-69) antibody
    • MDC(7-69) antibody
    • MGC34554 antibody
    • SCYA22 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 22 antibody
    • Small inducible cytokine subfamily A, member 22 antibody
    • Small-inducible cytokine A22 antibody
    • STCP 1 antibody
    • STCP1 antibody
    • Stimulated T cell chemotactic protein 1 antibody
    • Stimulated T-cell chemotactic protein 1 antibody
    see all

Anti-MDC antibody (ab9847) 使用論文

This product has been referenced in:
  • Döring C  et al. A novel immunohistochemical classifier to distinguish Hodgkin lymphoma from ALK anaplastic large cell lymphoma. Mod Pathol N/A:N/A (2014). Read more (PubMed: 24633193) »
  • Li YQ  et al. Tumor Secretion of CCL22 Activates Intratumoral Treg Infiltration and Is Independent Prognostic Predictor of Breast Cancer. PLoS One 8:e76379 (2013). IHC-P ; Human . Read more (PubMed: 24124553) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Colorectal)
Permeabilization Yes - 1x PBS with 0.3% triton-x-100
Specification Colorectal
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
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投稿 May 08 2015



ab9675 and ab9847 are not conjugated. If you would like to conjugate them to fluorochromes, we have easy kits which would allow you to do this. Please see: http://www.abcam.com/easylink

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"