製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MCU
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment: HHRTVHQRIA SWQNLGAVYC STVVPSDDVT VVYQNGLPVI SVRLPSRRER CQFTLKPISD SVGVFLRQLQ EEDRGIDRVA IYSPDGVRVA ASTGIDLLLL DDFKLV, corresponding to N terminal amino acids 47-152 of Human MCU (Uniprot ID = Q8NE86)

  • ポジティブ・コントロール
    • Human duodenum tissue. Human cell line U-2 OS.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Recommended fixative: PFA/Triton X-100

ターゲット情報

画像

  • ab121499 staining MCU in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Image shows moderate to strong granular cytoplasmic positivity in glandular cells

     

  • ab121499 staining MCU in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Image shows strong granular cytoplasmic positivity in glandular cells

  • Anti-MCU antibody (ab121499) + Human cell line A-549

    Predicted band size : 40 kDa
  • Immunofluorescent staining of Human cell line U-2 OS (fixed and permeabilized in PFA/Triton X-100) labeling MCU with ab121499 at 4µg/ml.
  • Immunohistochemical analysis of paraffin-embedded Human duodenum labeling MCU in glandular cells with ab121499 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 was performed.

参考文献

This product has been referenced in:
  • Cho B  et al. Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division. Nat Commun 8:15754 (2017). Read more (PubMed: 28598422) »
  • Oropeza-Almazán Y  et al. Small Interfering RNA Targeting Mitochondrial Calcium Uniporter Improves Cardiomyocyte Cell Viability in Hypoxia/Reoxygenation Injury by Reducing Calcium Overload. Oxid Med Cell Longev 2017:5750897 (2017). Read more (PubMed: 28337252) »

See all 6 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.3% TritonX-100
Specification
Spinal Cord
Blocking step
BSA + Normal Goat Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 07 2015

Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12& BIS-TRIS)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 20 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Loading amount
30 µg
Specification
Heart
Treatment
miRNA overexpression
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 27°C
Username

Dr. Samarjit Das

Verified customer

投稿 Apr 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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