Anti-MCPT4 抗体 (ab92368)

製品の概要

  • 製品名
    Anti-MCPT4 antibody
  • 製品の詳細
    Goat polyclonal to MCPT4
  • 由来種
    Goat
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性

    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    KLQKKAKETPSVN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 114 - 126 of Mouse MCPT4

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab92368 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-
    -Ile-5' bond of angiotensin I and the 'Phe-20-
    -Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-
    -His-9' bond of angiotensin I and the 'Phe-4-
    -Ala-5' and 'Tyr-10-
    -Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
  • 組織特異性
    Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.
  • 配列類似性
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mast cell protease 4 antibody
    • Mcp 4 antibody
    • Mcpt4 antibody
    • MCPT4_MOUSE antibody
    • MMCP 4 antibody
    • MMCP 4A antibody
    • MMCP 4B antibody
    • MMCP-4 antibody
    • MMCP4 antibody
    • MMCP4A antibody
    • MMCP4B antibody
    • MSMCP antibody
    • Myonase antibody
    • Serosal mast cell protease antibody
    see all

画像

  • Immunohistochemical staining of paraffin embedded Mouse Skeletal Muscle with ab92368 (3.75 µg/ml) . Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

プロトコール

参考文献

ab92368 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Permeabilization
No
Fixative
10% Formalin
Username

Abcam user community

Verified customer

投稿 Aug 26 2014

Thank you for contacting us.

Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide.

ELISA titre deter...

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Thank you for contacting us.

ab92368 is a Fast Track antibody. Fast-Track antibodies constitute a diverse group of products that have undergone variable degrees of characterization. They have all been affinity purified and show high titre valu...

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Thank you for contacting us regarding ab92368. As you may know, ab92368 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immun...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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