製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MCP5
  • アプリケーション
    適用あり: WB, ELISA, Neutralisingmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mMCP-5 (mouse Macrophage/Monocyte Chemotactic Protein-5).

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
Neutralising
  • 追加情報
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100.00 ng/ml), a concentration of 2.0 - 3.0 µg/ml of this antibody is required.

    ELISA:To detect mMCP-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

    Western Blot:To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • ターゲット情報

    • 機能
      Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
    • 組織特異性
      Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
    • 配列類似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-C motif chemokine 12 antibody
      • Ccl12 antibody
      • CCL12_MOUSE antibody
      • Macrophage/monocyte Chemotactic Protein antibody
      • MCP-1-related chemokine antibody
      • MCP-5 antibody
      • Monocyte chemoattractant protein 5 antibody
      • Monocyte chemotactic protein 5 antibody
      • OTTMUSP00000000792 antibody
      • Scya12 antibody
      • Small inducible cytokine A12 antibody
      • Small-inducible cytokine A12 antibody
      see all

    プロトコール

    参考文献

    ab9737 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for contacting Abcam. I am sorry that you are having trouble with ab9737. From what I have read about MCP5, it does have a predicted molecular weight of ~12kDa. I have a few questions/suggestions about the protocol that you are using: 1 - Doe...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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