製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    542
  • ミエローマ
    Sp2/0-Ag14
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 8.5 kDa.
    This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3µg/well; Substrate:TMB.
    A Western blot using an antibody concentration of 0.5-2µg/mL will allow visualization of 0.1µg/lane of human MCP-3.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • 配列類似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾
      O-glycosylated.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-C motif chemokine 7 antibody
      • Ccl7 antibody
      • CCL7_HUMAN antibody
      • Chemokine CC motif ligand 7 antibody
      • FIC antibody
      • MARC antibody
      • MCP-3 antibody
      • Monocyte chemoattractant protein 3 antibody
      • Monocyte chemotactic protein 3 antibody
      • NC28 antibody
      • RP23-350G1.4 antibody
      • SCYA6 antibody
      • SCYA7 antibody
      • Small-inducible cytokine A7 antibody
      see all

    プロトコール

    Anti-MCP3 antibody [542] (ab18693) 使用論文

    ab18693 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skin)
    Loading amount
    15 µg
    Specification
    Skin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
    Username

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    Verified customer

    投稿 Apr 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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