製品の概要

  • 製品名
    Anti-MCP3 antibody [542]
    MCP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [542] to MCP3
  • 特異性
    This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).

  • ポジティブ・コントロール
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    542
  • ミエローマ
    Sp2/0-Ag14
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 8.5 kDa.
    This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3µg/well; Substrate:TMB.
    A Western blot using an antibody concentration of 0.5-2µg/mL will allow visualization of 0.1µg/lane of human MCP-3.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • 配列類似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾
      O-glycosylated.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-C motif chemokine 7 antibody
      • Ccl7 antibody
      • CCL7_HUMAN antibody
      • Chemokine CC motif ligand 7 antibody
      • FIC antibody
      • MARC antibody
      • MCP-3 antibody
      • Monocyte chemoattractant protein 3 antibody
      • Monocyte chemotactic protein 3 antibody
      • NC28 antibody
      • RP23-350G1.4 antibody
      • SCYA6 antibody
      • SCYA7 antibody
      • Small-inducible cytokine A7 antibody
      see all

    プロトコール

    Anti-MCP3 antibody [542] (ab18693) 使用論文

    ab18693 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skin)
    Loading amount
    15 µg
    Specification
    Skin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
    Username

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    Verified customer

    投稿 Apr 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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