製品の概要

  • 製品名Anti-MCP2 antibody
    MCP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MCP2
  • アプリケーション適用あり: WB, Neutralising, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hMCP-2 (human Macrophage/Monocyte chemotactic protein-2)

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200µl of sterile water.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプunknown
  • 軽鎖の種類unknown
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100.0 ng/ml), a concentration of 12.0 - 16.0 µg/ml of this antibody is required
IHC-P Use a concentration of 0.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP2.

ターゲット情報

  • 機能Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • 組織特異性Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ccl8 antibody
    • CCL8_HUMAN antibody
    • HC14 antibody
    • MCP-2 antibody
    • MCP-2(6-76) antibody
    • Monocyte chemoattractant protein 2 antibody
    • Monocyte chemotactic protein 2 antibody
    • SCYA10 antibody
    • SCYA8 antibody
    • Small-inducible cytokine A8 antibody
    see all

Anti-MCP2 antibody 画像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal gastric mucosa, staining MCP2 with ab9671 at 0.75 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Staining was detected using DAB.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal gastric mucosa, staining MCP2 with ab9671 at 0.75 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Staining was detected using DAB.

Anti-MCP2 antibody (ab9671) 使用論文

This product has been referenced in:
  • Del Galdo F  et al. NF?B activation and stimulation of chemokine production in normal human macrophages by the gadolinium-based magnetic resonance contrast agent Omniscan: possible role in the pathogenesis of nephrogenic systemic fibrosis. Ann Rheum Dis 69:2024-33 (2010). IHC-P ; Human . Read more (PubMed: 20959327) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"