Anti-MCP1 抗体 [S101] (ab18678)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Neutralising
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    Neut: Use at a concentration of 0.5 µg/ml.
    WB: Use at a concentration of 0.1 - 5 µg/ml. At a concentration of 0.1 - 1.0 µg/mL the antibody will allow visualization of 0.1 µg/lane of human MCP1. Concentration of 5.0 µg/mL will allow visualization of 50 µg/lane of rabbit MCP1. Predicted molecular weight: 11 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
    • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Anti-MCP1 antibody [S101] (ab18678) 使用論文

    ab18678 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (20%)
    Sample Rabbit Tissue lysate - whole (aorta)
    Specification aorta
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
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    投稿 Jun 17 2013

    La forma no glicosilada de MCP-1 migra a 11KDa. Sin embargo, el peso molecular de MCP-1 varía debido a las glicosilaciones que se dan en las células. Estas bandas que observáis a 15-20KDa deben corresponder a las formas glicosilada...

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    Thank you for your enquiry. Rabbit reactivity was tested on rabbit homogenized thoracic aorta. Dilution: Antibody concentration of 5 µg/mL (1:200) will allow visualization of 50 µg/lane of total protein. I hope this information helps. Please ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"