製品の概要

  • 製品名
    Anti-MCP1 antibody [ECE.2]
    MCP1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ECE.2] to MCP1
  • 特異性
    The antibody reacts with murine JE / MCP-1.
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Synthetic peptide:

    LFKTASALRSSAPLNVKLTRKSEANASTT

    , corresponding to C terminal amino acids 102-130 of Mouse MCP1

  • 特記事項
    Monocyte chemotactic protein-1 (MCP-1) is also known as MCAF.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150. PubMed: 15829914
IHC-Fr Use at an assay dependent dilution. PubMed: 19920349
IHC-P 1/50.
WB 1/50.

ターゲット情報

  • 機能
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

画像

  • Detection of MCP-1 in mouse testis. ab8101 has been used in a 10x dilution, the magnification was 200x.

  • Immunohistochemical analysis of formalin-fixed murine duodenal tissue, staining MCP1 with ab8101.

参考文献

This product has been referenced in:
  • Stokes K  et al. The Circadian Clock Gene BMAL1 Coordinates Intestinal Regeneration. Cell Mol Gastroenterol Hepatol 4:95-114 (2017). Read more (PubMed: 28593182) »
  • Hirasawa M  et al. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration. J Biol Chem 291:7373-85 (2016). IHC . Read more (PubMed: 26839315) »

See all 11 Publications for this product

レビューと Q&A

Thank you for your reply.

I can confirm this antibody ab8101 is not conjugated. If an antibody is conjugated, this information is provided on the datasheet.

I can recommend to consider conjugating the antibody with a conjugate of ...

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Thank you for contacting us. Yes, this antibody has been tested and guaranteed for use in IHC. Please see the references for images.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Thank you for your enquiry. For a positive tissue control for ab8101, we recommend any vascular tissue, for instance spleen or lung. For antigen retrieval, we do not have information on which AR treatment, if any, is optimal for this antibody. We...

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Thank you for your enquiry. We're currently aware of one publication which featured the use of this antibody: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 91...

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Thank you for your question regarding ab8101. The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The antibody reacts with murine JE / MCP-1, but it has not yet been tested for use with paraffin embedded tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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