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Recombinant fragment, corresponding to amino acids 24/148 of Mouse MCP1(Peptide available as ab9901.)
JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages
Our Abpromise guarantee covers the use of ab9899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. To detect mJE by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mJE (100 ng/ml), a concentration of 4.0 - 6.0 µg/ml of this antibody is required.|
|WB||Use at an assay dependent concentration. Can be blocked with Recombinant mouse MCP1 protein (ab9901). To detect mJE by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab9899 has not yet been referenced specifically in any publications.