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Our Abpromise guarantee covers the use of ab9858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection.|
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.2 - 0.4 µg/ml.|
ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
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