製品の概要

  • 製品名Anti-MCP1 antibody
    MCP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MCP1
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Rat).

  • ポジティブ・コントロール
    • RAW 264.7 cells (mouse macrophage/monocyte adherent cell line) stimulated with LPS for 15 hours

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 21088249
ICC/IF Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 18 kDa.

ターゲット情報

  • 機能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody 画像

Anti-MCP1 antibody (ab7202) 使用論文

This product has been referenced in:
  • Riveiro-Naveira RR  et al. Resveratrol lowers synovial hyperplasia, inflammatory markers and oxidative damage in an acute antigen-induced arthritis model. Rheumatology (Oxford) 55:1889-900 (2016). Read more (PubMed: 27354682) »
  • Yunoki T  et al. Anti-oxidative nutrient rich diet protects against acute ischemic brain damage in rats. Brain Res 1587:33-9 (2014). Read more (PubMed: 25175837) »

See all 26 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Treatment 2mg/kg LPS 1h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 16 2012

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your note.



This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab7202. For your information, the internal reference note for...

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Thank you for your enquiry regarding ab7202 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

The protocol looks fine to me. It may wel...

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Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on th...

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Thank you for your call today and for your questions about ab7202. As we discussed, we recommend using a microwave antigen retrieval method with this antibody. However, if the antibody is still staining the wrong localization, please let me know a...

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Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (injred spinal cord tissue)
Specification injred spinal cord tissue
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. soo ryung jeong

Verified customer

投稿 Oct 01 2009

I'm sorry to hear the customer is having problems with his new vial of ab7202. Thank you for taking the time to fill in the questionnaire, I can see clearly that the protocol looks very good and the antibody should perform if the customer has previousl...

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I'm sorry to hear you are having problem with ab7102. We have not tested it in mouse aorta or have any reviews yet from customers using it in aorta. So please do let us know when your staining works and we will reward you with an Amazon gift voucher (a...

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Thank you for your phone call. There are no special conditions that need to be followed to use ab7202 in IHC-frozen sections and so I suggest fixing your sections with acetone as per our general IHC protocol for frozen sections (the protocol can be fou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"