製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MCP1
  • 特異性
    ab25124 reacts with both rat MCP1 and its mouse homolog JE.
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, Neutralising, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).
    Sequence:

    MQVSVTLLGL LFTVAACSIH VLSQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KRE

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Rat Kidney.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Neutralising Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 25 kDa.

ターゲット情報

  • 機能
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

画像

  • IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution

    Lane 1 : 40ug whole rat spinal cord protein lysate
    Lane 2 : 40ug whole mouse spinal cord protein lysate
    Lane 3 : Naive Rat
    Lane 4 : Sham 1
    Lane 5 : Sham 2
    Lane 6 : Injured 1
    Lane 7 : Injured 2
    Lane 8 : Injured 3

    Secondary
    Goat anti-Rabbit HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutesThis image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.

    See Abreview

参考文献

This product has been referenced in:
  • Sithu SD  et al. Atherogenesis and metabolic dysregulation in LDL receptor-knockout rats. JCI Insight 2:N/A (2017). IHC-P ; Rat . Read more (PubMed: 28469073) »
  • Sung Hsieh HH  et al. Evaluation of Salivary Cytokines for Diagnosis of both Trauma-Induced and Genetic Heterotopic Ossification. Front Endocrinol (Lausanne) 8:74 (2017). IHC-P ; Mouse . Read more (PubMed: 28484423) »

See all 14 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 14 2015

Application
Western blot
Sample
Human Recombinant protein (Recombinant protein)
Loading amount
0.2 µg
Specification
Recombinant protein
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2010

Thank you for your enquiry. We recommend that the azide be dialyzed out of the antibody preparation before any in vitro or in vivo studies.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Primary Mixed Cortical Cultures)
Specification
Primary Mixed Cortical Cultures
Permeabilization
No
Username

Ms. Nancy Nutile-McMenemy

Verified customer

投稿 Apr 12 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal Cord)
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Nancy Nutile-McMenemy

Verified customer

投稿 Dec 07 2005

Thank you for your enquiry. This antiserum was tested by western blotting using RAW cells treated with LPS for 15 hours. 10ug of lysate and 10ug of supernatant was loaded onto the gel. A prominent band of 25 kDa was detected. I hope this informat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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