The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.5µg for 105-8 cells in a total staining volume of 100µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Belongs to the intercrine beta (chemokine CC) family.
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Staining of 1-day LPS-activated normal Human peripheral blood mononuclear cells with ab91516 at 0.5 ?g (blue histogram), or FITC Armenian Hamster IgG isotype control at 0.5 ?g (green histogram). Cells in the monocyte gate were used for analysis.