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CHO expressed, recombinant mouse MCP-1.
Our Abpromise guarantee covers the use of ab21396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.|
|Flow Cyt||Use 0.5µg for 106 cells. in 100µl volume for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP1 producing cells within mixed cell populations.
ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. Samples need to be saponin-treated.|