製品の概要

  • 製品名Anti-MCP1 antibody [24822.11]
    MCP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [24822.11] to MCP1
  • アプリケーション適用あり: Neutralisingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human MCP-1 (rhMCP-1), expressed in E. coli.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising
  • 追加情報Neut: Use at a concentration of 0.1 µg/ml.

    Human MCP1 is tested for its ability to neutralize the chemoattractant activity of rhMCP1 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP1 that was present at a concentration just high enough to elicit a maximum response.

    In this bioassay, rhMCP1 was preincubated with various dilutions of the antibody for 1 hour at 37 °C in a 96-well microtiter plate. Then, the MCP1 antibody solution containing rhMCP1 at a final concentration of 0.1 µg/ml was transferred to the lower compartment of a 96-well chemotaxis chamber. The chemotaxis chamber was assembled using a PVP-free polycarbonate filter and the monocytes were added to the top chamber. The chamber was incubated for 90 minutes at 37 °C in a 5% CO2 humidified incubator. The chamber was disassembled and the filter fixed and stained. The optical density of the filter, which is proportional to the number of cells that migrated across the filter, was read with a microtiter plate reader set at 540 nm.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
    • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Anti-MCP1 antibody [24822.11] (ab10390) 使用論文

    ab10390 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab10390.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"