製品の概要

  • 製品名Anti-MCM8 antibody
    MCM8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MCM8
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: LRDAPEKTLA CMGLAIHQVL TKDLERHAAE LQAQEGLSND GETMVNVPHI, corresponding to N terminal amino acids 145-194 of Human MCM8

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 78, 82 kDa (predicted molecular weight: 93 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

ターゲット情報

  • 機能Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.
  • 組織特異性Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
  • 配列類似性Belongs to the MCM family.
    Contains 1 MCM domain.
  • 細胞内局在Nucleus. Localizes to nuclear foci and colocalizes with RAD51.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20orf154 antibody
    • dJ967N21.5 antibody
    • DNA helicase MCM8 antibody
    • DNA replication licensing factor MCM8 antibody
    • MCM8 antibody
    • MCM8_HUMAN antibody
    • MGC119522 antibody
    • MGC119523 antibody
    • MGC12866 antibody
    • MGC4816 antibody
    • Minichromosome maintenance 8 antibody
    • Minichromosome maintenance complex component 8 antibody
    • REC antibody
    see all

Anti-MCM8 antibody 画像

  • Anti-MCM8 antibody (ab66761) at 0.125 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

Anti-MCM8 antibody (ab66761) 使用論文

ab66761 has not yet been referenced specifically in any publications.

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