Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
配列類似性Belongs to the MCM family. Contains 1 MCM domain.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
All lanes : Anti-MCM7 antibody (ab92385) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 81 kDa
Exposure time : 3 minutes
Anti-MCM7 antibody (ab92385) 使用論文
has not yet been referenced specifically in any publications.