製品の概要

  • 製品名
    Anti-MCM7 antibody [47DC141]
    MCM7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [47DC141] to MCM7
  • アプリケーション
    適用あり: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Xenopus laevis
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    47DC141
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IP Use at 2 µg/mg of lysate.
WB 1/25 - 1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Incubate for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation. By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7.
Flow Cyt 1/20.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • 配列類似性
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDABP0042 antibody
    • CDC 47 antibody
    • CDC47 antibody
    • CDC47 homolog antibody
    • Cdc47, S. cerevisiae, homolog of antibody
    • DNA replication licensing factor MCM7 antibody
    • Homolog of S. cerevisiae Cdc47 antibody
    • MCM 2 antibody
    • MCM 7 antibody
    • MCM2 antibody
    • MCM2, formerly antibody
    • Mcm7 antibody
    • MCM7 minichromosome maintenance deficient 7 antibody
    • MCM7_HUMAN antibody
    • Minichromosome Maintainence 7 antibody
    • Minichromosome maintainence, S. cerevisiae, homolog of antibody
    • Minichromosome maintenance complex component 7 antibody
    • Minichromosome maintenance deficient 7 antibody
    • Minichromosome maintenance protein 7 antibody
    • P1.1 MCM3 antibody
    • P1.1-MCM3 antibody
    • P1CDC47 antibody
    • P85MCM antibody
    • PNAS 146 antibody
    • PNAS146 antibody
    • PPP1R104 antibody
    • Protein phosphatase 1, regulatory subunit 104 antibody
    see all

Anti-MCM7 antibody [47DC141] 画像

  • ab2360 - immunohistochemistry

    Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.

  • This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC.  Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.

    The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.

     
     
  • All lanes : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution

    Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
    Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.

    Secondary
    HRP conjugated Donkey anti-rabbit IgG
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab2360 staining MCM7 in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.

    See Abreview

  • Lane 1 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/50 dilution
    Lane 2 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
    Lane 3 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/500 dilution

    Lane 1 : Whole cell lysate prepared from SW780 cells
    Lane 2 : Whole cell lysate prepared from SW780 cells
    Lane 3 : Whole cell lysate prepared from SW780 cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview

    Gel run under denaturing conditions 4-12% gradient.

    See Abreview

  • ICC/IF image of ab2360 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLA cells stained with ab2360 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab2360 staining MCM7 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.

Anti-MCM7 antibody [47DC141] (ab2360) 使用論文

This product has been referenced in:
  • Bai G  et al. Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. PLoS Genet 12:e1005787 (2016). WB ; Mouse . Read more (PubMed: 26765334) »
  • Renaudin X  et al. Proteomic analysis reveals a FANCA-modulated neddylation pathway involved in CXCR5 membrane targeting and cell mobility. J Cell Sci 127:3546-54 (2014). Read more (PubMed: 25015289) »

See all 13 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3MEF)
Gel Running Conditions
Non-reduced Denaturing (7.5% acrylamide-Bis gel)
Loading amount
50000 cells
Treatment
by serum starvation for 48h , cells are synchronized at G0 phase and released with 5% FBS and collected at indicated time
Specification
3T3MEF
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
Yes - with wash - Buffer with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount
25 µg
Specification
SW780 bladder cancer
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

投稿 May 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bladder Cancer / Transitional cell Carcinoma)
Specification
Bladder Cancer / Transitional cell Carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

投稿 Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T (LANE 1 AND 2))
Loading amount
25 µg
Specification
293T (LANE 1 AND 2)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (Egg extracts)
Loading amount
12 µg
Specification
Egg extracts
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Jul 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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