製品の概要

  • 製品名
    Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]
    MCM5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CRCT5.1 (A2.7A3)] to MCM5
  • 由来種
    Mouse
  • 特異性
    Recognises human Mcm-5
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Bacterially expressed human Mcm5

  • ポジティブ・コントロール
    • Cervical Carcinoma Tissue
  • 特記事項
    Clone Number: CRCT5.1 (A2.7A3)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    CRCT5.1 (A2.7A3)
  • ミエローマ
    Sp2
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18693245

ターゲット情報

  • 配列類似性
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

画像

  • ab6154 staining MCM5 in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab6154 staining MCM5 in the HeLa cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde , permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal was used as the secondary antibody (1/200).

    See Abreview

参考文献

This product has been referenced in:
  • Munday DC  et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). Read more (PubMed: 25355874) »
  • Rakotomalala L  et al. Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy. J Biol Chem 283:28729-40 (2008). ICC/IF ; Mouse . Read more (PubMed: 18693245) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Jan 02 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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