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Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
Our Abpromise guarantee covers the use of ab17967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).|
|IP||Use a concentration of 5 µg/ml.
This antibody is efficient at pulling down the MCM2-7 complex.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
MCM5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MCM5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17967.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 97kDa; MCM5
ab17967, at 0.2