Anti-MCM4 (phospho S54) 抗体 (ab74014)
Key features and details
- Rabbit polyclonal to MCM4 (phospho S54)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-MCM4 (phospho S54) antibody
MCM4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM4 (phospho S54) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: African green monkey
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human MCM4 (phospho S54).
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ポジティブ・コントロール
- Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74014の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 96 kDa.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 96 kDa. |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 17217 Mouse
- SwissProt: P49717 Mouse
- Unigene: 1500 Mouse
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別名
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
画像
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All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours)
Lane 2 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab74014 は論文での使用が確認できていません。