製品の概要

  • 製品名Anti-MCM4 antibody [EPR5245]
    MCM4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5245] to MCM4
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • ポジティブ・コントロール
    • HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値) KD = 1.80 x 10 -11 M Learn more about KD
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5245
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/200000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • 配列類似性Belongs to the MCM family.
      Contains 1 MCM domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 19G antibody
      • AI325074 antibody
      • AU045576 antibody
      • CDC 21 antibody
      • CDC 54 antibody
      • CDC21 antibody
      • CDC21 homolog antibody
      • CDC21, S. pombe, homolog of antibody
      • CDC54 antibody
      • Cell division cycle 21, S. pombe, homolog of antibody
      • DNA replication licensing factor MCM 4 antibody
      • DNA replication licensing factor MCM4 antibody
      • hCdc 21 antibody
      • hCdc21 antibody
      • Homolog of S. pombe cell devision cycle 21 antibody
      • KIAA4003 antibody
      • mcdc21 antibody
      • MCM 4 antibody
      • MCM 4 minichromosome maintenance deficient 4 antibody
      • mcm4 antibody
      • MCM4 minichromosome maintenance deficient 4 antibody
      • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
      • MCM4_HUMAN antibody
      • Mcmd4 antibody
      • MGC33310 antibody
      • Minichromosome maintenance 4 antibody
      • Minichromosome maintenance complex component 4 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
      • Minichromosome maintenance deficient 4 antibody
      • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
      • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
      • mKIAA4003 antibody
      • NKCD antibody
      • NKGCD antibody
      • P1 CDC21 antibody
      • P1-CDC21 antibody
      • P1CDC21 antibody
      see all

    Anti-MCM4 antibody [EPR5245] 画像

    • All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Molt-4 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 97 kDa
    • Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-MCM4 antibody [EPR5245] (ab124836) 使用論文

    ab124836 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124836.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"