製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to MCM4
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-863 of Human MCM4 (AAH31061.1)

  • ポジティブ・コントロール
    • WB: placenta or HeLa cell lysate; IHC: tonsil tissue; ICC/IF: Hela cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • 配列類似性
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 19G antibody
    • AI325074 antibody
    • AU045576 antibody
    • CDC 21 antibody
    • CDC 54 antibody
    • CDC21 antibody
    • CDC21 homolog antibody
    • CDC21, S. pombe, homolog of antibody
    • CDC54 antibody
    • Cell division cycle 21, S. pombe, homolog of antibody
    • DNA replication licensing factor MCM 4 antibody
    • DNA replication licensing factor MCM4 antibody
    • hCdc 21 antibody
    • hCdc21 antibody
    • Homolog of S. pombe cell devision cycle 21 antibody
    • KIAA4003 antibody
    • mcdc21 antibody
    • MCM 4 antibody
    • MCM 4 minichromosome maintenance deficient 4 antibody
    • mcm4 antibody
    • MCM4 minichromosome maintenance deficient 4 antibody
    • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
    • MCM4_HUMAN antibody
    • Mcmd4 antibody
    • MGC33310 antibody
    • Minichromosome maintenance 4 antibody
    • Minichromosome maintenance complex component 4 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
    • Minichromosome maintenance deficient 4 antibody
    • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
    • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
    • mKIAA4003 antibody
    • NKCD antibody
    • NKGCD antibody
    • P1 CDC21 antibody
    • P1-CDC21 antibody
    • P1CDC21 antibody
    see all

画像

  • ab88546 at 10ug/ml staining MCM4 by immunofluorescence on HeLa cells.
  • ab88546 at 3ug/ml staining MCM4 on formalin-fixed, paraffin-embedded Human tonsil tissue.
  • All lanes : Anti-MCM4 antibody (ab88546) at 1 µg/ml

    Lane 1 : transfected 293T cells
    Lane 2 : non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 96 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Anti-MCM4 antibody (ab88546) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 96 kDa
  • Anti-MCM4 antibody (ab88546) at 1 µg/ml + Human placenta lysate at 50 µg

    Predicted band size : 96 kDa

参考文献

ab88546 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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