製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MCM4
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Chicken, Human
    交差が予測される動物種: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

ターゲット情報

  • 機能
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • 配列類似性
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 19G antibody
    • AI325074 antibody
    • AU045576 antibody
    • CDC 21 antibody
    • CDC 54 antibody
    • CDC21 antibody
    • CDC21 homolog antibody
    • CDC21, S. pombe, homolog of antibody
    • CDC54 antibody
    • Cell division cycle 21, S. pombe, homolog of antibody
    • DNA replication licensing factor MCM 4 antibody
    • DNA replication licensing factor MCM4 antibody
    • hCdc 21 antibody
    • hCdc21 antibody
    • Homolog of S. pombe cell devision cycle 21 antibody
    • KIAA4003 antibody
    • mcdc21 antibody
    • MCM 4 antibody
    • MCM 4 minichromosome maintenance deficient 4 antibody
    • mcm4 antibody
    • MCM4 minichromosome maintenance deficient 4 antibody
    • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
    • MCM4_HUMAN antibody
    • Mcmd4 antibody
    • MGC33310 antibody
    • Minichromosome maintenance 4 antibody
    • Minichromosome maintenance complex component 4 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
    • Minichromosome maintenance deficient 4 antibody
    • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
    • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
    • mKIAA4003 antibody
    • NKCD antibody
    • NKGCD antibody
    • P1 CDC21 antibody
    • P1-CDC21 antibody
    • P1CDC21 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 100 kDa

    Sample: Hela cell RIPA extract (60µg)

    Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.

    Detection: ECL.

     

    Sample: Hela cell RIPA extract (60µg)

    Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.

    Detection: ECL.

参考文献

This product has been referenced in:
  • Tenca P  et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007). Read more (PubMed: 17062569) »
  • Hochegger H  et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human, Chicken . Read more (PubMed: 17635936) »

See all 2 Publications for this product

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