Anti-MCM2 (phospho S40 + S41) 抗体 (ab84391)

製品の概要

  • 製品名
    Anti-MCM2 (phospho S40 + S41) antibody
    MCM2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MCM2 (phospho S40 + S41)
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • 免疫原

    Synthetic di-phosphorylated peptide corresponding to a sequence from human MCM2 surrounding Serines 40 and 41 (NP_004517.2).

  • ポジティブ・コントロール
    • Human colon adenocarcinoma and mouse squamous cell carcinoma tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab84391 is affinity purified using an epitope specific to MCM2 (phospho S40 + S41) immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • 配列類似性
      Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻訳後修飾
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    画像

    • ab84391, at a 1/250 dilution, staining MCM2 (phospho S40 + S41) in formalin fixed, paraffin embedded human colon adenocarcinoma tissue by Immunohistochemistry.
      1) Mock phosphatase treated section
      2) Calf intestinal phosphatase-treated section
      Detection: DAB staining.
    • ab84391, at a 1/250 dilution, staining MCM2 (phospho S40 + S41) in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue by Immunohistochemistry.
      1) Mock phosphatase treated section
      2) Calf intestinal phosphatase-treated section
      Detection: DAB staining.

    プロトコール

    参考文献

    ab84391 has not yet been referenced specifically in any publications.

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