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Immunogen was a synthetic phosphorylated peptide, which represented a portion of human MCM2 surrounding Serine32 (LocusLink ID 4171).
This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Our Abpromise guarantee covers the use of ab11897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/20000. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).|
Samples: Whole cell lysate (30
Samples: Whole cell lysate (30 µg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: ab1187 used at 0.1 µg/ml. After probing with the phospho-specific antibodies, ab4461 was used at 0.2 µg/ml to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.