Anti-MCM2 (phospho S27) 抗体 [EPR4119] (ab109459)


  • 製品名Anti-MCM2 (phospho S27) antibody [EPR4119]
    MCM2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4119] to MCM2 (phospho S27)
  • 特異性This antibody only detects MCM2 when phosphorylated at serine 27.
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 27 of Human MCM2.

  • ポジティブ・コントロール
    • HeLa cell lysate and HeLa cells. Human cervical carcinoma tissue. Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20ºC.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR4119
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab109459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 102 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • 配列類似性Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻訳後修飾Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 (phospho S27) antibody [EPR4119] 画像

    • All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilution

      Lane 1 : HeLa cell lysates untreated with alkaline phosphatase
      Lane 2 : HeLa cell lysates treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
    • ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
    • MCM2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) with ab109459 at 1/100 dilution.

      Exposue time:10 second.

    • ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.

    Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) 使用論文

    ab109459 has not yet been referenced specifically in any publications.

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