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RabMAb

Anti-MCM2 (phospho S108) 抗体 [EPR4121] (ab109271)

製品の概要

  • 製品名
    Anti-MCM2 (phospho S108) antibody [EPR4121]
    MCM2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4121] to MCM2 (phospho S108)
  • 特異性
    ab109271 only detects MCM2 phosphorylated at serine 108.
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Phospho specific peptide corresponding to residues surrounding Serine 108 of Human MCM2.

  • ポジティブ・コントロール
    • HeLa cell lysate; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4121
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • 配列類似性
      Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻訳後修飾
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    画像

    • All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 102 kDa
    • Immunofluorescent staining of HeLa cells using ab109271 at a dilution of 1/100.
    • MCM2 was immunoprecipitated from Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) with ab109271 at 1/80 dilution.

      Exposue time:10 second.

    参考文献

    ab109271 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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