製品の概要
-
製品名Anti-MCM2 antibody [SP85], prediluted
MCM2 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [SP85] to MCM2, prediluted
-
アプリケーション適用あり: IHC-P, Flow Cyt, WBmore details
-
種交差性交差種: Human
-
免疫原
Synthetic peptide within Human MCM2 aa 120-260 (internal sequence). The exact sequence is proprietary.
Database link: P49736 -
ポジティブ･コントロール
- Human rectal carcinoma tissue
製品の特性
-
製品の状態Prediluted
-
保存方法Shipped at 4°C. Store at +4°C.
-
バッファーPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6 -
Concentration information loading... -
精製度Protein A purified
-
ポリ/モノモノクローナル
-
クローン名SP85
-
アイソタイプIgG
-
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab95362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 | |
|---|---|---|---|
| IHC-P | 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min). | ||
| Flow Cyt | 1/100.
|
||
| WB | 1/100. |
ターゲット情報
-
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
-
配列類似性Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
-
細胞内局在Nucleus.
- Information by UniProt
-
参照データベース
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
-
別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Anti-MCM2 antibody [SP85], prediluted 画像
-
![Western blot - MCM2 antibody [SP85], prediluted (ab95362)](http://a.abcam.com/ps/products/95/ab95362/Images/MCM2-Primary-antibodies-ab95362-2.jpg)
Western blot - MCM2 antibody [SP85], prediluted (ab95362)Anti-MCM2 antibody [SP85] (ab95361) at 1/100 dilution + lysate prepared from HeLa cells
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
ab95361 used in the image is the undiluted version of ab95362. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [SP85], prediluted (ab95362)](http://a.abcam.com/ps/products/95/ab95362/Images/MCM2-Primary-antibodies-ab95362-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 antibody [SP85], prediluted (ab95362)Prediluted ab95362 staining MCM2 in formalin-fixed, paraffin-embedded Human rectal carcinoma tissue. -
![Flow Cytometry - Anti-MCM2 antibody [SP85], prediluted (ab95362)](http://a.abcam.com/ps/products/95/ab95362/Images/ab95362-266104-Flow-----5.jpg)
Flow Cytometry - Anti-MCM2 antibody [SP85], prediluted (ab95362)Flow cytometric analysis using rabbit anti-MCM2 (SP85) antibody ab95362 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue)
Anti-MCM2 antibody [SP85], prediluted (ab95362) 使用論文
ab95362 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"