製品の概要

  • 製品名Anti-MCM10 antibody
    MCM10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MCM10
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Orangutan非交差種: Xenopus laevis, Drosophila melanogaster
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

ターゲット情報

  • 機能Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
  • 配列類似性Belongs to the MCM10 family.
  • 発生段階Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
  • ドメインThe zinc finger-like domain binds a zinc ion and is involved in self-association.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ31133 antibody
    • HsMCM10 antibody
    • mcm10 antibody
    • MCM10 minichromosome maintenance deficient 10 antibody
    • MCM10_HUMAN antibody
    • Minichromosome maintenance protein 10 isoform 1 antibody
    • PRO2249 antibody
    • Protein MCM10 homolog antibody
    see all

Anti-MCM10 antibody 画像



  • Predicted band size : 110 kDa


    Sample: Hela cell lysate (1mg for IP).

    Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

    Detection: ECL.

Anti-MCM10 antibody (ab3733) 使用論文

This product has been referenced in:
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . Read more (PubMed: 24726359) »
  • Im JS  et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). Read more (PubMed: 19805216) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
Loading amount 20 µg
Specification HeLa cells and BJ human fibroblast cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jun 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"