製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MCM-BP
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human MCM-BP (NP_079110.1).

  • ポジティブ・コントロール
    • HeLa, 293T and Jurkat whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab124290 was affinity purified using an epitope specific to MCM-BP immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
  • 配列類似性
    Belongs to the MCMBP family.
  • 細胞内局在
    Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C10orf119 antibody
    • chromosome 10 open reading frame 119 antibody
    • MCM-binding protein antibody
    • MCM-BP antibody
    • mcmbp antibody
    • MCMBP_HUMAN antibody
    • Mini-chromosome maintenance complex-binding protein antibody
    • Minichromosome maintenance complex binding protein antibody
    see all

画像

  • All lanes : Anti-MCM-BP antibody (ab124290) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Exposure time : 3 minutes
  • ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 73 kDa. Exposure time: 30 seconds

参考文献

ab124290 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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