製品の概要

  • 製品名
    Anti-mCherry antibody [1C51]
    mCherry 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1C51] to mCherry
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 免疫原

    Recombinant full length His tagged mCherry purified from E. coli.

  • ポジティブ・コントロール
    • HEK293 crude extract transfected with pFin-EF1-mCherry; Hek293 cells transfected with mCherry.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.067% Sodium azide
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    1C51
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 28 kDa.
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

画像

  • Immunocytochemical analysis of HEK293 cells labelling mCherry which has been transfected into cells and labelled using ab125096 (with a red secondary). DAPI was used to stain DNA meaning only their nuclei are visualised in blue.

  • ab125096 staining mCherry in human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with BSA + Milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab125096 staining mCherry in smooth muscle cells in rat bladder by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Chen XW  et al. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis. Oncogene N/A:N/A (2017). Mouse . Read more (PubMed: 28481877) »
  • San Gil R  et al. Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells. Sci Rep 7:2387 (2017). Read more (PubMed: 28539657) »

See all 35 Publications for this product

レビューと Q&A


I can confirm that we have tested this antibody with DsRed and it works well.

Thank you for your patience. The originator of the antibody anti-mCherry ab125096 has confirmed that it cross-reacts with mTomato. Please let us know if you have any other questions.

Thank you for contacting us. I contacted the laboratory and have some data on two antibodies, the mCHerry antibody ab125096 should only have mild cross-reactivity with tomato and not with tag-RFP or GFP. This antibody as not yet been tested in IP, howe...

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Thank you for contacting us.

1. To our knowledge the ab125096 has not been tested in IHC-Fr yet and we can not guarantee the product to detect the mCherry in IHC-Fr. Regrettably do not offer any other mCherry antibody. However, if the customer...

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Thank you for your enquiry.

I can confirm that this antibody has been tested and is covered by the 6 month guarantee in ICC-IF. As the mcherry is not species related, it should detect the mcherry if you have an mcherry tagged recombinant pro...

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Thank you very much for your reply and for sending this information.

Is the antigen retrieval done in a pressure cooker or on a hotplate? 5 minutes might not be enough time to unmask the epitopes at normal pressure. I would recommend boiling ...

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Thank you very much for contacting us and letting us know about the trouble with these antibodies.

I am sorry to see that the results have been poor. I do have some additional questions so that I better understand the situation:

1) ...

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Thank you for contacting us.


I can confirm that, though this product does have the same image but this is not a XXXX product.

The price of Abcam products justifies the round of clock technical support and customer service we prov...

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Thank you for contacting us.


We know that the mCherry antibody does not bind GFP on blots, as we did test for that. The antibody picked up transfected mCherry in Hek293 in a whole cell extract without any problem. We saw a band at the e...

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Thank you for contacting us.

The antibody can be used in western blot and ICC/IF irrespective to species of sample.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informa...

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1-10 of 31 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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