Anti-MCEMP1 抗体 (ab121447)
Key features and details
- Rabbit polyclonal to MCEMP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCEMP1 antibody
MCEMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCEMP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to amino acids 111-187 of Human MCEMP1 (Q8IX19).
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ポジティブ・コントロール
- Human bone marrow; HEK293T cell MCEMP1 over-expression lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121447の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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組織特異性
Expressed specifically in mast cells. Found primarily in lung. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 199675 Human
- Omim: 609565 Human
- SwissProt: Q8IX19 Human
- Unigene: 709539 Human
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別名
- C19orf59 antibody
- Chromosome 19 open reading frame 59 antibody
- Mast cell expressed membrane protein 1 antibody
see all
画像
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ab121447, at 1/150 dilution, staining MCEMP1 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry, showing strong cytoplasmic positivity in bone marrow poietic cells.
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All lanes : Anti-MCEMP1 antibody (ab121447) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121447 は 1 報の論文で使用されています。
- Chen JX et al. Silence of long noncoding RNA NEAT1 exerts suppressive effects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation. IUBMB Life 71:956-968 (2019). PubMed: 30883005