Anti-MC-2 抗体 (ab87470)
Key features and details
- Rabbit polyclonal to MC-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MC-2 antibody
MC-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MC-2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Hamster, Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human MC-2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125889) -
ポジティブ・コントロール
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. -
組織特異性
Expressed in the brain but not in the melanoma cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 468599 Chimpanzee
- Entrez Gene: 101143231 Gorilla
- Entrez Gene: 4161 Human
- Entrez Gene: 17203 Mouse
- Omim: 600042 Human
- SwissProt: Q9TT23 Chimpanzee
- SwissProt: P33032 Human
- SwissProt: P41149 Mouse
see all -
別名
- MC-2 antibody
- MC2 antibody
- MC5 R antibody
see all
画像
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Lane 1 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)
All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesMC-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk (3%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab87470 は論文での使用が確認できていません。