製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MBOAT2
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence corresponding to amino acids 468-520 (TQRRKNTHEN IQLSQSKKFD EGENSLGQNS FSTTNNVCNQ NQEIASRHSS LKQ) of Human MBOAT2.

  • ポジティブ・コントロール
    • Human colon tissue; RT-4 and U-251 MG cell lysates; Human Liver lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Acyltransferase which mediates the conversion of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity). Catalyzes also the acylation of lysophosphatidic acid (LPA) into phosphatidic acid (PA) (LPAAT activity). Has also a very weak lysophosphatidylcholine acyltransferase (LPCAT activity). Prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.
  • 組織特異性
    Expressed in neutrophils.
  • パスウェイ
    Lipid metabolism; phospholipid metabolism.
  • 配列類似性
    Belongs to the membrane-bound acyltransferase family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1-acylglycerophosphate O-acyltransferase antibody
    • 1-acylglycerophosphoethanolamine O-acyltransferase antibody
    • LPAAT antibody
    • LPCAT4 antibody
    • LPEAT antibody
    • LPLAT 2 antibody
    • Lyso-PA acyltransferase antibody
    • Lyso-PE acyltransferase antibody
    • Lysophosphatidic acid acyltransferase antibody
    • Lysophosphatidylethanolamine acyltransferase antibody
    • Lysophospholipid acyltransferase 2 antibody
    • MBOA2_HUMAN antibody
    • Mboat2 antibody
    • Membrane bound O acyltransferase domain containing 2 antibody
    • Membrane-bound O-acyltransferase domain-containing protein 2 antibody
    • O acyltransferase (membrane bound) domain containing 2 antibody
    • O-acyltransferase domain-containing protein 2 antibody
    • OACT2 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121453 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121453, at 1/1500 dilution, staining MBOAT2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-MBOAT2 antibody (ab121453) at 1/250 dilution

    Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human Liver lysate
    Lane 6 : Human Tonsil lysate

参考文献

ab121453 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab121453.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録