製品の概要

  • 製品名Anti-MBD4 antibody
    MBD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MBD4
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide corresponding to amino acids 541 to 554 of mouse MBD4 conjugated to KLH via an N terminal added cysteine residue. The sequence is conserved in human.

  • ポジティブ・コントロール
    • Nuclear extracts of K562 cell line.
  • 特記事項Storage in frost free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified using the immunizing peptide immobilized on agarose.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa). Staining of the MBD4 band in immunoblotting is specifically inhibited by the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
    • 配列類似性Contains 1 MBD (methyl-CpG-binding) domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3 N(4) ethenocytosine glycosylase antibody
      • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
      • G/T mismatch glycosylase antibody
      • G/U mismatch glycosylase antibody
      • MBD 4 antibody
      • MBD4 antibody
      • MBD4_HUMAN antibody
      • MED 1 antibody
      • MED1 antibody
      • Methyl CpG binding domain protein 4 antibody
      • Methyl CpG binding endonuclease 1 antibody
      • Methyl CpG binding protein MBD4 antibody
      • Methyl-CpG-binding domain protein 4 antibody
      • Methyl-CpG-binding endonuclease 1 antibody
      • Methyl-CpG-binding protein MBD4 antibody
      • Mismatch specific DNA N glycosylase antibody
      • Mismatch-specific DNA N-glycosylase antibody
      • Putative methyl CpG binding protein antibody
      see all

    Anti-MBD4 antibody 画像

    • Lane 1 : Anti-MBD4 antibody (ab12187) at 1 µg/ml (Rabbit anti-MBD4)
      Lane 2 : Anti-MBD4 antibody (ab12187) at 0.5 µg/ml (Rabbit anti-MBD4)

      Lane 1 : K-562 cells
      Lane 2 : K-562 cells

      Secondary
      Goat anti-rabbit IgG, peroxidase and a chemiluminescent substrate

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    Anti-MBD4 antibody (ab12187) 使用論文

    This product has been referenced in:
    • Pettersen HS  et al. UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21745813) »
    • Bertoni C  et al. Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides. Nucleic Acids Res 37:7468-82 (2009). WB ; Mouse . Read more (PubMed: 19854937) »

    See all 3 Publications for this product

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    There are currently no Abreviews or Questions for ab12187.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"