製品の概要

  • 製品名
    Anti-MBD3 antibody - Carboxyterminal end
    MBD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MBD3 - Carboxyterminal end
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the C-terminal region of human MBD3.

  • ポジティブ・コントロール
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and transfected/ non-transfected 293 cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • 配列類似性
    Contains 1 MBD (methyl-CpG-binding) domain.
  • 細胞内局在
    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI181826 antibody
    • AU019209 antibody
    • MBD 3 antibody
    • Mbd3 antibody
    • MBD3: methyl CpG binding domain protein 3 antibody
    • MBD3_HUMAN antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD3 antibody
    • Methyl-CpG-binding domain protein 3 antibody
    • Methyl-CpG-binding protein MBD3 antibody
    see all

画像

  • All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : MBD3 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71777 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

参考文献

This product has been referenced in:
  • Hata K  et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Read more (PubMed: 24336734) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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