Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - MBD3 antibody (ab91458)
All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 33 kDa
Exposure time : 30 seconds
Immunoprecipitation - MBD3 antibody (ab91458)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458. 20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.